期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 19, 期 12, 页码 1542-1546出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.0904.04033
关键词
Carotenoids; zeaxanthin; crtX gene; zeaxanthin glucosyltransferase; Parcoccus haeundaensis
资金
- Korean Government (MOEHRD, Basic Research Promotion Fund) [KRF-2007-314-F00039]
Zeaxanthin glucosyltransferase (CrtX) mediates the formation of zeaxanthin to zeaxanthin diglucoside. Here, we report cloning of the crtX gene responsible for zeaxanthin diglucoside biosynthesis from Paracoccus haeundaensis and the production of the corresponding carotenoids in transformed cells carrying this gene. An expression plasmid containing the crtX gene (pSTCRT-X) was constructed, and Escherichia coli cells containing this plasmid produced the recombinant protein of approximately 46 kDa. Biosynthesis of zeaxanthin diglucoside was obtained when the plasmid pSTCRT-X was co-transformed into E. coli containing the pET-44a(+)-CrtEBIYZ carrying crtE, crtB, crtI, crtY, and crtZ genes required for zeaxanthin beta-D-diglucoside biosynthesis.
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