期刊
JOURNAL OF MICROBIOLOGY
卷 56, 期 8, 页码 593-599出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-018-8273-2
关键词
cytomegalovirus; Epstein-Barr virus; polyomavirus BK; multiplex qPCR; transplant recipients
类别
资金
- Ministry of Health & Welfare, Republic of Korea [HI15C2955]
- Leaders in INdustry-university Cooperation + Project - Ministry of Education
- Leaders in INdustry-university Cooperation + Project - National Research Foundation of Korea
- Basic Science Research Program through the NRF - Ministry of Science, ICT & Future Planning [NRF-2015M3A9B5030157]
Transplant recipients are more susceptible to bacterial and viral infections. Cytomegalovirus (CMV), Epstein-Barr virus (EBV), and polyomavirus BK (BK) are risk factors for graft dysfunction. All three of them are latent viruses that can cause serious disease in immunocompromised patients. Mainly qualitative PCR tests are required for diagnosis and quantitative monitoring, which are used to follow the response to transplantation. We developed a multiplex real-time PCR (qPCR) method to detect these viruses during blood screenings of transplant recipients. We also validated analytical and clinical performance tests using the developed multiplex qPCR. The limit of detection (LOD) was 100, 125, and 183 copies/ml for CMV, EBV, and BK, respectively. These results had high linearity (R-2 = 0.997) and reproducibility (CV range, 0.95-2.38%, 0.52-3.32%, and 0.31-2.45%, respectively). Among 183 samples, we detected 8 samples that were positive for CMV, while only 6 were positive for EBV, and 3 were positive for BK. Therefore, the viral infection prevalence in transplant candidates was 4.40% for CMV, 3.29% for EBV, and 1.64% for BK. This multiplex qPCR method should be used widely for diagnosing and monitoring latent viral infections in transplant recipients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据