期刊
JOURNAL OF MICROBIOLOGY
卷 52, 期 11, 页码 940-947出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-014-4517-y
关键词
Aspergillus nidulans; beta-1,3-glucan synthase gene; transcription; StuA; EMSA; sGFP-fusion
类别
资金
- Chungnam National University [2014-0796-01]
The temporal and spatial regulation of beta-1,3-glucan synthesis plays an important role in morphogenesis during fungal growth and development. Northern blot analysis showed that the transcription of fksA, the gene encoding beta-1,3-glucan synthase in Aspergillus nidulans, was cell-cycle-dependent and increased steadily over the duration of the vegetative period, but its overall expression during the asexual and sexual stages was fairly constant up until the time of transcription cessation. In an A. nidulans strain mutated in the eukaryotic bHLH-like APSES transcription factor stuA1, the transcriptional level of fksA, and consequently the content of alkali-insoluble cell wall beta-glucan, significantly increased at the conidial chain formation and maturation stage. Electrophoretic mobility shift assays revealed that StuA was bound to StREs (StuA Response Elements) on the fksA promoter region. Promoter analysis with sGFP-fusion constructs also indicated the negative regulation of fksA expression by StuA, especially during asexual development. Taken together, these data suggest that StuA plays an important role in cell wall biogenesis during the development of A. nidulans, by controlling the transcription level of fksA.
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