期刊
JOURNAL OF MICROBIOLOGY
卷 50, 期 4, 页码 693-697出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-012-2234-y
关键词
poly(3-hydroxypropionate); malonyl-CoA reductase; propionyl-coenzyme A synthetase; polyhydroxyalkanoate synthase; recombinant E. coli
类别
资金
- Director Innovation Foundation of QIBEBT
- CAS [Y112141105]
- National Natural Scientific Foundation of China [21106170]
- Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences [KSCX2-EW-G-13]
- Qingdao Applied Basic Research Program [12-1-4-9-(5)-jch]
Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.
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