期刊
JOURNAL OF MICROBIOLOGY
卷 49, 期 4, 页码 558-561出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-011-1052-y
关键词
mcr-A gene; methanogens; Murrah buffaloes; phylogenetics
类别
资金
- Natioanl Fund for Basic and Strategic research in Agriculture (NFBSRA)
- Indian Council of Agriculture Research (ICAR), Government of India
The aim of the present study was to decipher the diversity of methanogens in rumen of Murrah buffaloes so that effective strategies can be made in order to mitigate methane emission from these methanogens. In the present study diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India was evaluated by using mcr-A gene library obtained from the pooled PCR product from four animals and by using MEGA4 software. A total of 104 clones were examined, revealing 26 different mcr-A gene sequences or phylotypes. Of the 26 phylotypes, 16 (64 of 104 clones) were less than 97% similar to any of the cultured strain of methanogens. Seven clone sequences were clustered with Methanomicrobium mobile and three clone sequences were clustered with Methanobrevibacter gottschalkii during the phylogenetic analysis. Uncultured group of methanogens comes out to he the major component of the methanogens community structure in Murrah buffaloes. Methanomicrobium phylotype conies out to be major phylotype among cultured methanogens followed by Methanobrevibacter phylotype. These results help in making effective strategies to check the growth of dominant methanogenic communities in the rumen of this animal which in turn help in the reduction of methane emission in the environment and ultimately helps us in fighting with the problem of global warming.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据