期刊
JOURNAL OF MICROBIOLOGY
卷 49, 期 2, 页码 315-319出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-011-1028-y
关键词
Porphyromonas gingivalis; rpoB; species-specific; qPCR primers
类别
资金
- Ministry of Education, Science and Technology [2009-0076542]
- National Research Foundation of Korea [2009-0076542] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Species-specific quantitative real-time PCR (qPCR) primers were developed for the detection of Porphyromonas gingivalis. These primers, Pg-F/Pg-R, were designed based on the nucleotide sequences of RNA polymerase beta-subunit gene (rpoB). Species-specific amplicons were obtained from the tested P. gingivalis strains but not in any of the other strains (46 strains of 46 species). The qPCR primers could detect as little as 4 fg of P. gingivalis chromosomal DNA. These findings suggest that these qPCR primers are suitable for applications in epidemiological studies.
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