期刊
JOURNAL OF MICROBIOLOGY
卷 46, 期 4, 页码 441-447出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-008-0054-x
关键词
Pseudomonas aeruginosa; virulence factors; subinhibitory concentration of antibiotics; gene regulation
类别
资金
- NSFC [30470095, 306111211520]
- State Major Basic Research Program [2004CCA01700]
- AHFMR
Recent studies have shown that subinhibitory antibiotics play important roles in regulating bacterial genes including virulence factor genes. In this study, the expression of 13 secreted virulence related gene clusters of Pseudomonas aeruginosa, an important opportunistic pathogen, was examined in the presence of subinhibitory concentrations of 4 antibiotics: vancomycin, tetracycline, ampicilin and azithromycin. Activation of gene expression was observed with phzA1, rhlAB, phzA2, lasB, exoY, and exoS. Subinhibitory concentrations of vancomycin resulted in snore than 10-fold increase of rhlAB and phzA2 transcription. Both rhamnolipid production and pyocyanin production were significantly elevated, correlating phenotypes with the increased transcription. P. aeruginosa swarming and swimming motility also increased. Similar results were observed with subinhibitory tetracycline, azithromycin and ampicillin. These results indicate that the antibiotics at low concentrations can up-regulate virulence factors and therefore influence bacterial pathogenesis.
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