期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 152, 期 -, 页码 165-172出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2018.08.005
关键词
B. subtilis; Biofilm; Cross-section; SEM; Filter
资金
- Helmholtz Space Life Sciences Research School (SpaceLife)
- German Aerospace Center (DLR) of the Helmholtz Association
- DGE (Deutsche Gesellschaft fur Elektronenmikroskopie)
Biofilms have been intensively investigated over the past decades. Bacillus subtilis is able to form highly structured colony biofilms, and as one of the most studied Gram-positive model organisms, has helped to decipher the complex genetic regulation of biofilms. Several methods have been developed to analyze the architecture of biofilms. In this paper, we describe a method which allows the analysis of the internal structures of biofilms by scanning electron microscopy (SEM). The method uses a modified freeze-fracturing of chemically fixed biofilm to generate defined, well-preserved fractures at millimeter-scale which allows to analyze systematically the internal biofilm structure from macro- to nano-scale.
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