Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR

The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of alpha- and beta-diversity with the MOTHUR software. (C) 2014 Elsevier B.V. All rights reserved.