期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 102, 期 -, 页码 66-68出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2014.04.010
关键词
L-asparaginase; Phenol red; Bromothymol blue; Methyl red
Fungi are well known to produce various industrial enzymes and secondary metabolites with different colours. Fungi producing L-asparaginase enzyme are conventionally screened on medium containing phenol red (PR). The contrast between enzyme-hydrolysed zone and unhydrolysed L-asparagine is not very evident and distinct in medium containing PR and bromothymol blue (BB) due to coloured secondary metabolite production. Thus, PR and BB limit and affect the detection and screening method. In the present investigation, an improved method for screening is reported by comparing with PR and BB, wherein methyl red (MR) is incorporated as pH indicator. The enzyme activity was distinctly observed (red and light-yellow) in MR incorporated medium compared to PR and BB. (C) 2014 Elsevier B.V. All rights reserved.
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