期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 93, 期 3, 页码 203-205出版社
ELSEVIER
DOI: 10.1016/j.mimet.2013.03.013
关键词
Chimera detection; Chimera formation; Environmental samples; PCR temperatures
Chimeric sequences falsely increase the apparent diversity within samples. To examine chimera formation in PCR products from environmental DNA, clone libraries were prepared using different ramp speeds to reach the designated temperatures for each step of the PCR program. Slowing the thermocycler ramp speed to 1 degrees C s(-1) reduced chimera formation. (C) 2013 Elsevier B.V. All rights reserved.
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