期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 92, 期 3, 页码 307-309出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2012.12.018
关键词
Staphylococcus aureus; MTT assay; HUVECs; Damage; Ea.hy926; A549
资金
- Foundation for Research at the Medical Faculty, University of Zurich, Switzerland
- Matching Funds of the Clinical Trials Center, University Hospital Zurich, Switzerland
We established the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess damage induced by Staphylococcus aureus in human umbilical vein endothelial cells (HUVECs). The MIT assay was comparable to the previously published (51)chromium release assay. MTT reduction by intracellular S. aureus was negligible and therefore permits assessment of S. aureus induced EC damage. (c) 2012 Elsevier B.V. All rights reserved.
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