期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 91, 期 1, 页码 165-170出版社
ELSEVIER
DOI: 10.1016/j.mimet.2012.08.003
关键词
H2S; Hydrogen sulfide; Fermentation; High throughput
资金
- Laffort Australia
- Industry Partnership grant
- University of Western Sydney
- Australia's grapegrowers and winemakers through their investment agency the Grape and Wine Research and Development Corporation
- Australian Government
An in situ high throughput method for the detection of H2S during fermentation was developed. The method utilizes a redox reaction in which sulfide ion reduces methylene blue, leading to its decolourisation. Incorporation of methylene blue into the fermentation media allows real-time detection of H2S during fermentation and the generation of an H2S production profile. Kinetic parameters extracted from the H2S production Profile can be used to characterise genetic factors affecting H2S production and differentiate between environmental conditions affecting it. The method, validated here for Saccharomyces cerevisiae, is suited for high throughput screening purposes by virtue of its simplicity and the ability to detect H2S in micro-scale fermentations. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据