期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 86, 期 2, 页码 266-273出版社
ELSEVIER
DOI: 10.1016/j.mimet.2011.04.018
关键词
Bacteria; Biofilm; Gene expression; Polymerase chain reaction (PCR); Viable but non-culturable (VBNC)
资金
- NSERC (Canada)
Viable but non-culturable (VBNC) bacteria are common in nutrient poor and/or stressed environments as planktonic cells and biofilms. This article discusses approaches to researching VBNC bacteria to obtain knowledge that is lacking on their gene expression while in the VBNC state, and when they enter into and then recover from this state, when provided with the necessary nutrients and environmental conditions to support growth and cell division. Two-dimensional gel electrophoresis of proteins, global gene expression, reverse-transcription polymerase chain reaction (PCR) analysis and sequencing by synthesis coupled with data on cell numbers, viability and species present are central to understanding the VBNC state. (C) 2011 Elsevier B.V. All rights reserved.
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