期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 83, 期 2, 页码 254-256出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.08.012
关键词
Membrane potential; Staphylococcus aureus; Microtiter; Assay
We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC(2)(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds. (c) 2010 Elsevier B.V. All rights reserved.
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