期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 82, 期 2, 页码 187-191出版社
ELSEVIER
DOI: 10.1016/j.mimet.2010.05.011
关键词
Nanofilaments; Nanowires; Scanning electron microscopy; Shewanella oneidensis MR-1
资金
- ONR [NRL/JA/7330-10-334]
Nanofilament production by Shewanella oneidensis MR-1 was evaluated as a function of lifestyle (planktonic vs. sessile) under aerobic and anaerobic conditions using different sample preparation techniques prior to imaging with scanning electron microscopy. Nanofilaments could be imaged on MR-1 cells grown in biofilms or planktonically under both aerobic and anaerobic batch culture conditions after fixation, critical point drying and coating with a conductive metal. Critical point drying was a requirement for imaging nanofilaments attached to planktonically grown MR-1 cells, but not for cells grown in a biofilm. Techniques described in this paper cannot be used to differentiate nanowires from pill or flagella. Published by Elsevier B.V.
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