期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 80, 期 2, 页码 215-216出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2009.12.002
关键词
Transformation; Nanopiercing; Yoshida effect; Sepiolite; E. coli; Acinetobacter; Yersinia
Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar. (C) 2010 Elsevier B.V. All rights reserved.
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