期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 83, 期 2, 页码 194-201出版社
ELSEVIER
DOI: 10.1016/j.mimet.2010.08.021
关键词
Bimolecular fluorescence complementation; Protein-protein interaction; Yellow fluorescent protein; Saccharomyces cerevisiae
资金
- Ministry of Education, Science and Technology, Republic of Korea [FPR08A1-060, MG-11-2008-09-004-00]
Most of the biological processes are carried out and regulated by dynamic networks of protein-protein interactions. In this study, we demonstrate the feasibility of the bimolecular fluorescence complementation (BiFC) assay for in vivo quantitative analysis of protein-protein interactions in Saccharomyces cerevisiae. We show that the BiFC assay can be used to quantify not only the amount but also the cell-to-cell variation of protein-protein interactions in S. cerevisiae. In addition, we show that protein sumoylation and condition-specific protein-protein interactions can be quantitatively analyzed by using the BiFC assay. Taken together, our results validate that the BiFC assay is a very effective method for quantitative analysis of protein-protein interactions in living yeast cells and has a great potential as a versatile tool for the study of protein function. (C) 2010 Elsevier B.V. All rights reserved.
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