期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 76, 期 3, 页码 316-319出版社
ELSEVIER
DOI: 10.1016/j.mimet.2008.12.003
关键词
Real-time PCR; qPCR; Quantification; Mismatch; Genetic instability
资金
- Vienna Science and Technology Fund (WWTF), Austria [LS231]
- Austrian Christian Doppler Research Society
Comparison of a broad range of characteristics of real-time PCR amplification curves yielded only slight alterations for low numbers of mismatches in the primer binding regions, resulting in a quantification error up to 63.12%. The effects were more pronounced for mismatches in the probe binding region and resulted in a quantification error up to 33%. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据