期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 78, 期 1, 页码 71-78出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2009.05.002
关键词
Als3; Als family; Candida albicans; Cell-surface glycoprotein; Monoclonal antibody
资金
- National Institute of Dental and Craniofacial Research, National Institutes of Health [DE14158]
- National Center for Research Resources, National Institutes of Health [C06 RR16515-01]
Two monoclonal antibodies (MAbs) were raised against the Candida albicans cell-surface glycoprotein Als3 using the N-terminal domain of the protein as the immunogen. ELISA was used to demonstrate the specificity of the MAbs for the Als3 fragment, but not for the corresponding N-terminal domain fragments from other proteins in the Als family. The anti-Als3 MAbs immunolabeled the surface of germ tubes from a diverse collection of wildtype C albicans isolates, but did not label yeast cells, an als3 Delta/als3 Delta deletion mutant strain, nor isolates of other Candida species associated with human disease. Als3 was visualized readily in fresh and formalin-fixed, paraffin-embedded kidney tissue from a murine model of candidiasis. The anti-Als3 MAbs were also useful for immunogold electron microscopy and Western blotting. Both MAbs blocked C albicans adhesion to vascular endothelial cells and buccal epithelial cells. These versatile MAbs are a valuable addition to the reagents available to study C. albicans cell surface dynamics and interaction of the fungus with host cells. (C) 2009 Elsevier B.V. All rights reserved.
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