期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 73, 期 2, 页码 195-198出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2008.02.013
关键词
site-directed mutagenesis; inverse PCR; ligase-free; 5'-adapter
Modifying the Site-directed, Ligase-Independent Mutagenesis (SLIM) protocol from a single reaction mode to a two-reaction mode enables highly efficient mutagenesis of plasmid constructs that exceed 8kb. This modified approach reduces the complexity of the PCR step and is optimised for generation of heteroduplexes from long PCR products. The two-reaction mode SLIM has 92% efficiency. (c) 2008 Elsevier B.V. All rights reserved.
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