Simulation of protein ultrafiltration using CFD: Comparison of concentration polarisation and fouling effects with filtration and protein adsorption experiments

The crossflow ultrafiltration of bovine serum albumin (BSA) solutions is studied by experimental tests and corresponding transient computational fluid dynamics (CFD) simulations. Experimental data collected includes total flux as a function of time, as well as final protein deposition dependant on feed volume flow, pH and protein concentration. A CFD solver is developed to simulate these data taking varying physical properties and fouling by a surface adsorption into account. The model equations and boundary conditions are documented and the physical property data available are reviewed and examined in detail. An excellent agreement of experiment and simulation can be achieved for a defined standard case. The response to the operating conditions is qualitatively correct however the concentration polarisation is under predicted in the simulation. Based on these findings an outlook is given on modelling strategies for better prediction of the complex transport phenomena for filtration separation of macromolecular solutions. (C) 2009 Elsevier B.V. All rights reserved.