期刊
JOURNAL OF MEMBRANE BIOLOGY
卷 247, 期 11, 页码 1161-1179出版社
SPRINGER
DOI: 10.1007/s00232-014-9711-7
关键词
Xylose; XylE structure; GLUT1; Facilitated diffusion; Docking
资金
- King's College London
Structural changes and xylose docking to eight conformers of Escherichia Coli XylE, a xylose transporter similar to mammalian passive glucose transporters GLUTs, have been examined. Xylose docks to inward and outward facing conformers at a high affinity central site (K (i) 4-20 A mu M), previously identified by crystallography and additionally consistently docks to lower affinity sites in the external and internal vestibules (K (i) 12-50 A mu M). All these sites lie within intramolecular tunnels and cavities. Several local regions in the central transmembrane zone have large positional divergences of both skeleton carbon C alpha positions and side chains. One such in TM 10 is the destabilizing sequence G388-P389-V390-C391 with an average RMSD (4.5 +/- A 0.4 ). Interchange between conformer poses results in coalescence of tunnels with adjacent cavities, thereby producing a transitory channel spanning the entire transporter. A fully open channel exists in one inward-facing apo-conformer, (PDB 4ja4c) as demonstrated by several different tunnel-finding algorithms. The conformer interchanges produce a gated network within a branched central channel that permits staged ligand diffusion across the transporter during the open gate periods. Simulation of this model demonstrates that small-scale conformational changes required for sequentially opening gate with frequencies in the ns-mu s time domain accommodate diffusive ligand flow between adjacent sites with association-dissociation rates in the mu s-ms domain without imposing delays. This current model helps to unify the apparently opposing concepts of alternate access and multisite models of ligand transport.
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