期刊
JOURNAL OF MEMBRANE BIOLOGY
卷 246, 期 3, 页码 215-230出版社
SPRINGER
DOI: 10.1007/s00232-012-9523-6
关键词
Calcium sensitive channels; Embryonic stem cells; Mechanosensitive channels
资金
- Ministerio de Educacion y Ciencia [SAB2006-0211]
- National Cancer Institute
- Department of Defense
- Fundacion Progreso y Salud
- Consejeria de Salud
- Junta de Andalucia [PI-0022/2008, CTS-6505, INP-2011-1615-900000]
- Consejeria de Innovacion Ciencia y Empresa
- Instituto de Salud Carlos III [Red TerCel-RD06/0010/0025, PI10/00964]
- Ministry of Health and Consumer Affairs [TRA-120]
Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca2+ permeant cation channels (MscCa) and large conductance (200 pS) Ca2+-sensitive K+ (BKCa2+) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BKCa2+ channel is more highly expressed in hESCs compared with mESCs (similar to 50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca2+-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca2+] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.
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