期刊
JOURNAL OF MEMBRANE BIOLOGY
卷 237, 期 2-3, 页码 79-91出版社
SPRINGER
DOI: 10.1007/s00232-010-9308-8
关键词
Purinergic receptor physiology in epithelia; Renal physiology; Ion channel; Potassium ion channel; Volume regulation in epithelial cells
资金
- Wellcome Trust
- University of Sheffield
Although previous studies have provided evidence for the expression of P2X receptors in renal proximal tubule, only one cell line study has provided functional evidence. The current study investigated the pharmacological properties and physiological role of native P2X-like currents in single frog proximal tubule cells using the whole-cell patch-clamp technique. Extracellular ATP activated a cation conductance (P2X(f)) that was also Ca(2+)-permeable. The agonist sequence for activation was ATP = alpha beta-MeATP > BzATP = 2-MeSATP, and P2X(f) was inhibited by suramin, PPADS and TNP-ATP. Activation of P2X(f) attenuated the rundown of a quinidine-sensitive K(+) conductance, suggesting that P2X(f) plays a role in K(+) channel regulation. In addition, ATP/ADP apyrase and inhibitors of P2X(f) inhibited regulatory volume decrease (RVD). These data are consistent with the presence of a P2X receptor that plays a role in the regulation of cell volume and K(+) channels in frog renal proximal tubule cells.
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