期刊
JOURNAL OF MEDICINAL FOOD
卷 16, 期 11, 页码 984-991出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/jmf.2013.0050
关键词
Calabrian pine; hydroxyl radical; 15-lipoxygenase; proanthocyanidins; RP-HPLC-DAD-ESI-MS; superoxide anion radical; taxifolin
资金
- Cyprus Research Promotion Foundation (CRPF) [KY-POY/0407/03]
- National Authority for Scientific Research in Romania (Bilateral Cooperation) [311/2009]
- [POSDRU/88/1.5/S/58965]
A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The total phenolics and proanthocyanidins in each extract were quantified by spectrophotometric methods; the polyphenolic profile was analyzed by RP-HPLC-DAD-ESI-MS. All extracts were tested with regard to their ability to scavenge free radicals (ABTS radical cation, superoxide and hydroxyl radicals), reduce ferric ions, and inhibit 15-lipoxygenase. P. brutia bark extracts had high phenolic contents (303.79 +/- 7.34-448.90 +/- 1.39mg/g). Except diethyl ether extract, all other extracts contained proanthocyanidins ranging from 225.79 +/- 3.94 to 250.40 +/- 1.44mg/g. Several polyphenols were identified by RP-HPLC-DAD-ESI-MS: taxifolin in diethyl ether extract, a taxifolin-O-hexoside, catechin, procyanidin dimers, and trimers in ethyl acetate extract. Except diethyl ether extract, all other extracts were effective scavengers of superoxide and hydroxyl radicals (EC50=33.5 +/- 1.1-54.93 +/- 2.85 g/mL and 0.47 +/- 0.06-0.6 +/- 0.0mg/mL, respectively). All extracts had noticeable 15-lipoxygenase inhibitory effects (EC50=22.47 +/- 0.75-34.43 +/- 2.25 g/mL). We conclude that P. brutia bark is very promising for the dietary supplements industry due to its high free radical scavenging and 15-lipoxygenase inhibitory effects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据