期刊
JOURNAL OF MEDICAL VIROLOGY
卷 86, 期 12, 页码 2065-2069出版社
WILEY
DOI: 10.1002/jmv.23907
关键词
adenovirus; viability; fomites; hospital; biomarker
类别
资金
- Brazilian National Council for Scientific and Technological Development (CNPq)
- Oswaldo Cruz Institute (IOC-Fiocruz)
- Program of Research Excellence (PROEP)
- Carlos Chagas Filho Foundation for Research Support of the state of Rio de Janeiro (FAPERJ)
- Instituto Oswaldo Cruz-IOC, Cellular and Molecular Biology Program
The monitoring of environmental microbial contamination in healthcare facilities may be a valuable tool to determine pathogens transmission in those settings; however, such procedure is limited to bacterial indicators. Viruses are found commonly in those environments and are rarely used for these procedures. The aim of this study was to assess distribution and viability of a human DNA virus on fomites in an Adult Intensive Care Unit of a private hospital in Rio de Janeiro, Brazil. Human adenoviruses (HAdV) were investigated in 141 fomites by scraping the surface area and screening by quantitative PCR (qPCR) using TaqMan (R) System (Carlsbad, CA). Ten positive samples were selected for virus isolation in A549 and/or HEp2c cell lines. A total of 63 samples (44.7%) were positive and presented viral load ranging from 2.48x10(1) to 2.1x10(3) genomic copies per millilitre (gc/ml). The viability was demonstrated by integrated cell culture/nested-PCR in 5 out of 10 samples. Nucleotide sequencing confirmed all samples as HAdV and characterized one of them as specie B, serotype 3 (HAdV-3). The results indicate the risk of nosocomial transmission via contaminated fomites and point out the use of HAdV as biomarkers of environmental contamination. J. Med. Virol. 86:2065-2069, 2014. (c) 2014 Wiley Periodicals, Inc.
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