期刊
JOURNAL OF MEDICAL VIROLOGY
卷 84, 期 6, 页码 906-911出版社
WILEY
DOI: 10.1002/jmv.23281
关键词
viral diagnosis; nasopharyngeal samples; bronchoalveolar fluids; throat; high throughput
类别
The aim of the study was to evaluate the MagNA Pure 96 (TM) nucleic acid extraction system using clinical respiratory specimens for identifying viruses by qualitative real-time PCR assays. Three extraction methods were tested, that is, the MagNA Pure LC (TM), the COBAS Ampliprep (TM), and the MagNA Pure 96 (TM) with 10-fold dilutions of an influenza A(H1N1)pdm09 sample. Two hundred thirty-nine respiratory specimens, 35 throat swabs, 164 nasopharyngeal specimens, and 40 broncho-alveolar fluids, were extracted with the MagNA Pure 96 (TM) and the COBAS Ampliprep (TM) instruments. Forty COBAS Ampliprep (TM) positive samples were also tested. Real-time PCRs were used to identify influenza A and influenza A(H1N1)pdm09, rhinovirus, enterovirus, adenovirus, varicella zoster virus, cytomegalovirus, and herpes simplex virus. Similar results were obtained on RNA extracted from dilutions of influenza A(H1N1)pdm09 with the three systems: the MagNA Pure LC (TM), the COBAS Ampliprep (TM), and the MagNA Pure 96 (TM). Data from clinical respiratory specimens extracted with the MagNA Pure 96 (TM) and COBAS Ampliprep (TM) instruments were in 98.5% in agreement (P?
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