期刊
JOURNAL OF MEDICAL PRIMATOLOGY
卷 38, 期 -, 页码 8-16出版社
WILEY
DOI: 10.1111/j.1600-0684.2009.00370.x
关键词
live attenuated SIV; protective immunity; simian immunodeficiency virus; T-cell activation
资金
- NCRR NIH HHS [P51 RR000168-370038, P51 RR000168, P51 RR00168] Funding Source: Medline
- NIAID NIH HHS [P01 AI071306-01A1, R01 AI062412, P01 AI071306, U19AI067854-04, R01 AI062412-05] Funding Source: Medline
Background Pathogenic HIV and SIV infections characteristically deplete central memory CD4+ T cells and induce chronic immune activation, but it is controversial whether this also occurs after vaccination with attenuated SIVs and whether depletion or activation of CD4+ T-cell play roles in protection against wild-type virus challenge. Methods Rhesus macaques were vaccinated with SIVmac239 Delta nef and quantitative and phenotypic polychromatic flow cytometry analyses were performed on mononuclear cells from blood, lymph nodes and rectal biopsies. Results Animals vaccinated with SIVmac239 Delta nef demonstrated no loss of CD4+ T cells in any tissue, and in fact CCR5+ and CD28+CD95+ central memory CD4+ T cells were significantly increased. In contrast, CD4+ T-cell numbers and CCR5 expression significantly declined in unvaccinated controls challenged with SIVmac239. Also, intracellular Ki67 increased acutely as much as 3-fold over baseline in all tissues after SIVmac239 Delta nef vaccination then declined following primary infection. Conclusion We demonstrated in this study that SIVmac239 Delta nef vaccination did not deplete CD4+ T cells but transiently activated and expanded the memory cell population. However, increases in numbers and activation of memory CD4+ T cells did not appear to influence protective immunity.
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