期刊
JOURNAL OF MEDICAL MICROBIOLOGY
卷 61, 期 1, 页码 137-141出版社
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/jmm.0.036046-0
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资金
- Bundesministerium fur Bildung und Forschung (BMBF), Germany, Klinische Forschergruppe Infektiologie [01KI0771]
- Veterans Affairs Merit Review Program
- National Institutes of Health [AI072219-05, AI063517-07]
- Geriatric Research Education and Clinical Center [VISN 10]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI063517, R01AI072219] Funding Source: NIH RePORTER
We have investigated the reproducibility of DiversiLab rep-PCR fingerprints between two laboratories with the aim of determining if the fingerprints and clustering are laboratory-specific or portable. One-hundred non-duplicate Acinetobacter baumannii isolates were used in this study. DNA isolation and rep-PCR were each performed separately in two laboratories and rep-PCR patterns generated in laboratory A were compared with those from laboratory B. Twelve A. baumannii isolates processed in laboratory A showed >= 98% pattern similarity with the corresponding 12 isolates tested in laboratory B and were considered identical. Sixty-four isolates showed 95-97.9% similarity with their corresponding isolates. Twenty-three isolates showed 90-94% similarity with the corresponding isolates, while one isolate showed only 87.4% similarity. However, intra-laboratory clustering was conserved: isolates that clustered in laboratory A also clustered in laboratory B. While clustering was conserved and reproducible at two different laboratories, demonstrating the robustness of rep-PCR, interlaboratory comparison of individual isolate fingerprints showed more variability. This comparison allows conclusions regarding clonality to be reached independent of the laboratory where the analysis is performed.
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