Identification of Genes Differentially Expressed During Heat Shock Treatment in Aedes aegypti

Temperature is important for mosquito development and physiological response. Several gene genes of heat shock protein (HSP) families are known to be expressed in mosquitoes and may be crucial ill responding to stress induced by elevated temperature. Suppression subtractive hybridization (SSH) was used to identify target transcripts to beat shock treatment ill female Aedes aegypti. Subtraction was performed ill both directions enriching, for cDNAs differentially expressed between it non-heat shock control and heat shock treatment. Heat shock treatment of female Ae. aegypti was carried out for I h at 42 degrees C. Clones from differentially expressed genes were evaluated by sequencing. Target transcripts up-regulated by heat shock included five different HSP gene families and 27 other genes, such its cytochrome o oxidase, serine-type endopeptidase, and glutamyl aminopeptidase. Additionally, some novel genes, cytoskeleton and ribosomal genes, were found to be differentially expressed, and three novel up-regulated sequences belonging to it low-abundance class of transcripts Were obtained. Up-regulated/down-regulated transcripts from heat shock treatment were further confirmed and quantified by quantitative real-time polymerase chain reaction (PCR). High temperatures can alter the gene expression of a vector mosquito population, and further characterization of these differentially expressed genes will provide information useful ill understanding the genetic response to heat shock treatment, which can be used to develop novel approaches to genetic control.