The circadian rhythm of expression of Bmal1 and Cry1 in peripheral blood mononuclear cells of preterm neonates

Objective. To determine circadian rhythms of circadian genes in the peripheral blood mononuclear cells in preterm neonates. Study design. Ten premature neonates, gestational age from 30 to 31 weeks were recruited. Infants with birth asphyxia, RDS, apnea, malformation, infection or haemolytic diseases were excluded. At 7AM and 7PM on the 1st day, 7th day and 14th day of hospitalisation, peripheral venous blood was obtained from the premature babies and real-time RTPCR was used to determine the expression of Bmal1 and Cry1. Results. The expression of bmal1 did not have statistical difference between 7AM and 7PM on the 1st day, 7th day and 14th day. Neither did the cry1. Conclusion. There was no circadian rhythm of bmal1 and cry1 in PBMC of preterm neonates in our NICU.