The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation

The adhesion and proliferation of human fetal osteoblasts, hFOB 1.19, on micro arc oxidized (MAO) gamma titanium aluminide (gamma TiAl) surfaces were examined in vitro. Cells were seeded on MAO treated gamma TiAl disks and incubated for 3 days at 33.5 A degrees C and subsequently for 7 days at 39.5 A degrees C. Samples were then analyzed by scanning electron microscopy (SEM) and alkaline phosphatase assay (ALP) to evaluate cell adhesion and differentiation, respectively. Similar Ti-6Al-4V alloy samples were used for comparison. Untreated gamma TiAl and Ti-6Al-4V disks to study the effect of micro arc oxidation and glass coverslips as cell growth controls were also incubated concurrently. The ALP Assay results, at 10 days post seeding, showed significant differences in cell differentiation, with P values < 0.05 between MAO gamma TiAl and MAO Ti-6Al-4V with respect to the corresponding untreated alloys. While SEM images showed that hFOB 1.19 cells adhered and proliferated on all MAO and untreated surfaces, as well as on glass coverslips at 10 days post seeding, cell differentiation, determined by the ALP assay, was significantly higher for the MAO alloys.