期刊
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
卷 23, 期 8, 页码 1991-2000出版社
SPRINGER
DOI: 10.1007/s10856-012-4666-7
关键词
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资金
- EU European Regional Development Fund, PolyMed [TEAM/2008-2/6]
- National Science Centre [2011/01/N/ST5/05544]
Novel polymeric hydrogel scaffolds for corneal epithelium cell culturing based on blends of chitosan with some other biopolymers such as hydroxypropylcellulose, collagen and elastin crosslinked with genipin, a natural substance, were prepared. Physicochemical and biomechanical properties of these materials were determined. The in vitro cell culture experiments with corneal epithelium cells have indicated that a membrane prepared from chitosan-collagen blend (Ch-Col) provided the regular stratified growth of the epithelium cells, good surface covering and increased number of the cell layers. Ch-Col membranes are therefore the most promising material among those studied. The performance of Ch-Col membranes is comparable with that of the amniotic membrane which is currently recommended for clinical applications.
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