期刊
PATHOLOGY RESEARCH AND PRACTICE
卷 211, 期 12, 页码 973-981出版社
ELSEVIER GMBH
DOI: 10.1016/j.prp.2015.10.002
关键词
alpha 5 beta 1; alpha 6 beta 4; alpha V beta 3; lntegrins; Fucosylation; Haptoglobin; Ovarian cancer
类别
资金
- S0008-FONSEC SSA/IMSS/ISSSTE [115617]
- CONACYT, Mexico
- CONACYT fellowship [301143]
Integrins are adhesion molecules whose expression is upregulated during different cellular processes such as adhesion, growth, proliferation, migration, survival and differentiation, all of which are involved in neoplastic development. Several reports have linked the overexpression of integrins with epithelial ovarian cancer (EOC). Furthermore, fucosylated haptoglobin (Hp) isoforms with antioxidant activity and synthesized primarily in the liver have also been associated with various types of cancer, including ovarian cancer. Here, we determined the level of expression of three integrin heterodimers (alpha 5 beta 1, alpha 6 beta 4, and alpha V beta 3) and fucosyltated Hp in two different settings: cell cultures and biopsies from ovarian cancer patients. On the one hand, integrin heterodimers were analyzed in the ovarian cancer cell line (SKOV-3), two primary cultures (INCan017 and INCan019) and a tumor derived from INCan017 (T-017) by Western blot. Statistical analysis was performed using one-way ANOVA. The SKOV-3 cell line, INCan017 and INCan019 primary cultures, and the T-017 tumor showed increased expression patterns of the alpha 5, alpha V, beta l, beta 3, and beta 4 integrin subunits when compared with healthy ovary tissue. We then analyzed the expression pattern of the integrin subunits as well as the fucosylated Hp in biopsies from patients with different his-totypes of EOC by immunofluorescence. alpha 5 beta 1 and alpha 6 beta 4 integrins were expressed by 90% of the samples, whereas 80% expressed the alpha V beta 3 integrin. Furthermore, Hp, fucosylated or not, was present at high levels in most biopsies. In fact, there was a statistical correlation between the expression of integrins or Hp and the presence of the disease given that alpha 5 beta 1, alpha 6 beta 4, and alpha V beta 3 integrins, Hp, fucosylated Hp and additional fucosylation state of proteins were highly expressed in biopsies of EOC histotypes when compared with healthy ovarian tissue. However, the statistical analysis showed no association of the presence of integrins, Hp or fucosylation with clinical or pathological characteristics of EOC patients. These results suggest that increased expression of these molecules and of the fucosylation modification are characteristics of the malignant process itself. Therefore, these molecules may be promising therapeutic targets in patients with this type of neoplasia. (C) 2015 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据