期刊
JOURNAL OF MATERIALS CHEMISTRY
卷 22, 期 25, 页码 12568-12573出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2jm31582c
关键词
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资金
- NSF of China [20935005, 21105104, 20905070]
- National Basic Research Program of China [2011CB935800, 2010CB933502]
- Chinese Academy of Science [KJCX2-EW-N06-01]
In this work, an efficient approach for targeting and detecting cancer cells has been developed through the design of the assembly of fluorescent carbon nanodots and folic acid (C-dots-FA), which is endocytosible by the overexpressed folate receptor (FR) molecule. The fluorescent C-dots were prepared by a facile microwave pyrolysis method, but their surfaces were passivated with 4,7,10-trioxa-1,13-tridecanediamine, so that active amino groups could be engineered for the further conjugation with FA. The uptake of the designed C-dots-FA by HeLa cancer cells, as revealed by confocal laser scanning microscopy, is via receptor-mediated endocytosis, which is further confirmed by competition experiments as well as a comparative study with FR-negative MCF-7 cells. The proposed method shows excellent biocompatibility, and, most notably, its applicability to discriminating FR-positive cancer cells from normal cells has been successfully demonstrated by culturing and analyzing the first model cell mixture of NIH-3T3 and HeLa cells, which makes it of great potential for cancer diagnosis studies.
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