期刊
JOURNAL OF MATERIALS CHEMISTRY
卷 21, 期 35, 页码 13470-13475出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1jm12098k
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资金
- National Natural Science Foundation of China (NSFC) [20875054, 90813014]
A ratiometric fluorescent pH probe, 4-carboxylaniline-5-chlorosalicylaldehyde Schiff base (1) was synthesized via a facile reaction and used for pH sensing in live cells. While exhibiting weak fluorescence when dispersed in solution, 1 displayed aggregation-induced emission enhancement (AIEE) characteristics in its aggregate/solid state, as a result of the restriction of free intramolecular rotation of a C-N bond and the non-planar configuration in the aggregate/solid state. The integration of hydroxyl and carboxyl groups provided 1 with a ratiometric fluorescent response to pH based on AIEE and the pH-dependent spectral characteristics of compound 1, with proton dissociation constants pK(a1) and pK(a2) of 4.8 +/- 0.1 and 7.4 +/- 0.1, respectively. The probe exhibited a significant fluorescence color change from orange to green with an intensity ratio (I-516 (nm)/I-559 nm) enhanced when the pH increased from 5.0 to 7.0 in aqueous solution. Confocal fluorescence imaging of intracellular pH through ratiometric response was successfully achieved in live HepG2 cells. The results demonstrate that probe 1 is a good candidate for monitoring pH fluctuations in live cells with good selectivity, stability, and excellent membrane permeability.
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