4.3 Article

Poly(epsilon-caprolactone)-graft-poly(2-(N, N-dimethylamino) ethyl methacrylate) nanoparticles: pH dependent thermo-sensitive multifunctional carriers for gene and drug delivery

期刊

JOURNAL OF MATERIALS CHEMISTRY
卷 20, 期 33, 页码 6935-6941

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/c0jm00506a

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资金

  1. 863 Program [2009AA03Z313]
  2. National Natural Science Foundation of China [30772007, 30970784]
  3. National Grand Program on Key Infectious Disease Control [2008ZX10001-015-10]
  4. National Key Basic Research Program of China [2009CB930200]

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pH-dependent temperature-sensitive poly(epsilon-caprolactone)-graft-poly(2-(dimethylamino) ethyl methacrylate) (PCL-g-PDMAEMA), a kind of degradable, amphiphilic, cationic copolymer, was synthesized. PCL-g-PDMAEMA was self-assembled into core-shell nanoparticles with an ultralow critical association concentration at about 8.1 x 10(-4) g L(-1). It was found that PCL-g-PDMAEMA nanoparticles were able to simultaneously entrap hydrophobic paclitaxel and load DNA. Hydrophobic drug paclitaxel, loaded by PCL-g-PDMAEMA NPs, could be released faster in an acidic environment than in a neutral environment, and PCL-g-PDMAEMA NPs showed a comparable in vitro gene transfection efficiency to Lipofectamine 2000. In addition, the gene transfection efficiency was enhanced by the addition of 5% serum. Besides, confocal microscopic measurements indicated that PCL-g-PDMAEMA nanoparticles/DNA polyplexes could escape from the endosome and release the payloads effectively in cytoplasm. These results suggest PCL-g-PDMAEMA has great potential for achieving the synergistic effect of drug and gene therapies in vivo.

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