Cell up-take control of gold nanoparticles functionalized with a thermoresponsive polymer

Surface decoration of gold nanoparticles with thermoresponsive polymers endows a temperature tunable colloidal system switchable for enhanced intracellular up-take. Gold nanoparticles (AuNP, 18 +/- 11 nm-diameter) produced by laser ablation synthesis in liquid solution were surface coated with thermoresponsive thiol terminated poly-N-isopropylacrylamide-co-acrylamide co-polymer possessing a lower critical solution temperature (LCST) at 37 degrees C. Under selected conditions about 3800 polymer chains were conjugated per particle. The polymer coated nanoparticles were found to display thermosensitive properties, as in solution they exhibited reversible aggregation/deaggregation above and below the LCST, respectively. Cell culture studies showed that the polymer decorated AuNP were located into human breast adenocarcinoma MCF7 cells treated at 40 degrees C (12000 AuNP/cell) with more than 80-fold greater up-take compared to cells treated at 34 degrees C with the same particles (140 AuN/cell). This difference is attributable to a switching' of the polymer coating to a globule state at 37 degrees C and an increased hydrophobicity of the particles with a simultaneous loss of the 'stealth' properties of the polymer coating. By contrast, cell up-take of uncoated AuNP (about 6000 AuNP/cell) did not depend on the incubation temperature. These data show that good control of the AuNP cell up-take can be obtained with the new polymer-gold nanoconjugates, and suggest that these systems might find use for targeting cells in vitro by a small temperature change or in vivo in body sites, such as inflamed or tumour tissues, where a temperature variation is already present.