期刊
PARASITOLOGY RESEARCH
卷 114, 期 3, 页码 1225-1228出版社
SPRINGER
DOI: 10.1007/s00436-015-4312-7
关键词
Schistosoma japonicum; Schistosomiasis; Zoonoses; Diagnosis
类别
资金
- Japan Society for the Promotion of Science [24405044, 23689024]
- Japan Science and Technology Agency [AS232Z00111F]
- Japan Society for the Promotion of Science Foreign Postdoctoral Grant [P14088]
- Grants-in-Aid for Scientific Research [23689024, 24405044] Funding Source: KAKEN
The zoonotic characteristic of the human parasite Schistosoma japonicum infecting a significant number of wild and domestic animals highlights the need to develop a unified surveillance in multiple host species for a strengthened schistosomiasis control. It has been shown in several studies that water buffaloes and dogs are considered important reservoirs in the transmission of the schistosome parasite to humans. Recombinant antigens like thioredoxin peroxidase-1 (SjTPx-1) and tandem repeat proteins (Sj1TR, Sj7TR) have been shown to be good diagnostic antigens individually in humans, water buffaloes, and dogs in previous studies. Mixing these antigens together in a cocktail-ELISA might not only improve their diagnostic potentials but rather produce a multi-host species detection means for zoonotic schistosomiasis. In this study, we aimed to develop and optimize cocktail-ELISA by testing different combinations of these recombinant antigens in humans, water buffaloes, and dogs. As compared with the diagnostic potential calculated for each of the three recombinant antigens used, their combination has presented improved specificities, positive predictive values, and kappa values. Using samples collected from various endemic areas in the Philippines, results showed that the combination of SjTPx-1/Sj7TR/Sj1TR has the highest sensitivity in humans (84.1 %), water buffaloes, and dogs (80 %) and specificity (100 %) in all host species. This study therefore suggests the use of cocktail-ELISA in improving the zoonotic surveillance in schistosomiasis endemic areas.
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