期刊
PARASITOLOGY RESEARCH
卷 114, 期 4, 页码 1607-1610出版社
SPRINGER
DOI: 10.1007/s00436-015-4343-0
关键词
Anopheles atroparvus; Diseases; Lynx pardinus; Non-invasive blood sampling; Parasites
类别
资金
- Spanish Ministry of Science and Innovation [CGL2012-30759, CGL2006-10853/BOS, CGL2010-21540/BOS]
- Junta de Andalucia [RNM157, RNM6400]
- European Commission EuroWestNile FP7 Project [261391]
- Consejeria de Medio Ambiente of the Junta de Andalucia
- Juan de la Cierva contract
Host identification from mosquito blood meals has been routinely used to identify the feeding preferences of insects in studies on transmission of vector-borne pathogens. Here, we identified for the first time the susceptibility of the endangered Iberian lynx (Lynx pardinus) to the attack of a wild mosquito female, the mosquito Anopheles atroparvus. Furthermore, we used 11 microsatellite markers to test for the utility of vertebrate DNA isolated from insect blood meals for individual identification of wildlife. Only the three smallest markers were successfully amplified; however, this genotype did not match with any of the previously genotyped individuals in southern Spain. These results support the use of DNA from mosquito blood meals as a non-invasive source of DNA and a powerful tool on epidemiological and conservation biology studies. However, as may be the case of other non-invasive sampling methods, the utility of this technique is probably limited by the quantity and quality of vertebrate DNA.
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