期刊
JOURNAL OF MASS SPECTROMETRY
卷 49, 期 7, 页码 639-645出版社
WILEY
DOI: 10.1002/jms.3385
关键词
mass spectrometry; ion suppression effect; induced electrosonic spray ionization; charge state distribution; ionization efficiency
资金
- National Natural Science Foundation of China [21205111]
- Recruitment Program of Global Experts (Thousand Young Talent Program)
In this study, alleviation of ion suppression effect in sonic spray ionization mass spectrometry (SSI-MS) was investigated. Ion suppression effect was firstly compared between electrospray ionization (ESI) and conventional SSI, and more severe ion suppression effect was observed with SSI. Ion suppression effect of SSI was also found difficult to be alleviated by simply optimizing major parameters. Alternatively, we found that with the assistance of an alternating current (AC) voltage with low amplitude, the ion suppression effect was greatly alleviated (comparable with conventional ESI). That AC voltage was applied outside the SSI spray tip, and no direct contact between the electrode and spray solution was necessary. Besides the alleviation of the ion suppression effect, this newly-developed method, termed as induced electrosonic spray ionization (IESSI), appeared to preserve similar charge state distribution with SSI for protonated cytochrome c, hemoglobin, and bradykinin. IESSI could also obtain significantly improved ion intensities (similar to 1000-fold over conventional SSI). In addition, tolerance of concentrated salts for IESSI-MS was investigated through the analysis of cytochrome c in the presence of concentrated sodium chloride (NaCl) or ammonium acetate (NH4OAc). Copyright (C) 2014 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据