期刊
JOURNAL OF MASS SPECTROMETRY
卷 47, 期 11, 页码 1473-1481出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.3108
关键词
imaging mass spectrometry; sub-cellular; sub-micron spatial resolution; transmission geometry; single-cell imaging
资金
- National Institute of General Medical Sciences from the National Institutes of Health [8 P41 GM103391-02, 5P41RR031461-02, 5R01 GM058008-13]
- Department of Defense [W81XWH-05-1-0179]
- Aegis Sciences Corporation
The need of cellular and sub-cellular spatial resolution in laser desorption ionization (LDI)/matrix-assisted LDI (MALDI) imaging mass spectrometry (IMS) necessitates micron and sub-micron laser spot sizes at biologically relevant sensitivities, introducing significant challenges for MS technology. To this end, we have developed a transmission geometry vacuum ion source that allows the laser beam to irradiate the back side of the sample. This arrangement obviates the mechanical/ion optic complications in the source by completely separating the optical lens and ion optic structures. We have experimentally demonstrated the viability of transmission geometry MALDI MS for imaging biological tissues and cells with sub-cellular spatial resolution. Furthermore, we demonstrate that in conjunction with new sample preparation protocols, the sensitivity of this instrument is sufficient to obtain molecular images at sub-micron spatial resolution. Copyright (c) 2012 John Wiley & Sons, Ltd.
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