期刊
JOURNAL OF MASS SPECTROMETRY
卷 47, 期 6, 页码 720-726出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.3006
关键词
orotic acid; mass spectrometry; atmospheric pressure chemical ionization; collision-induced dissociation; ion-molecule reactions; hydrogen-deuterium exchange
资金
- Ministry of Education, Youth and Sports of the Czech Republic [ME10013]
- Operational Program Research and Development for Innovations - European Regional Development Fund [CZ.1.05/2.1.00/03.0058]
- Czech Science Foundation [P206/12/1150]
- Institute of Microbiology of the ASCR, v.v.i. [RVO61388971]
Atmospheric pressure chemical ionization is known for producing unusual artifacts of the ionization process in some cases. In this work, processes occuring in atmospheric pressure chemical ionization/MS of orotic acid that afforded ions accompanying protonated and deprotonated orotic acid molecules in the spectra were studied. Two processes ran in parallel in the ion source: decarboxylation of neutral orotic acid and collision-induced dissociation of its protonated or deprotonated form. A procedure discerning pre-ionization decomposition and post-ionization dissociation by manipulating ion source parameters was proposed. Experiments with isotopically labeled solvents confirmed ion-molecule reactions of the product of collision-induced dissociation of protonated orotic acid with solvent molecules in the ion source and even under vacuum in the ion trap. Copyright (C) 2012 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据