期刊
JOURNAL OF MASS SPECTROMETRY
卷 46, 期 6, 页码 561-567出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.1925
关键词
Gal beta 1,4Fuc epitope; planaria; N-glycan; methylhexose; mass spectrometry
资金
- Austrian Fonds zur Forderung der wissenschaftlichen Forschung [P18847, P21946]
- Austrian Science Fund (FWF) [P 21946] Funding Source: researchfish
- Austrian Science Fund (FWF) [P18847, P21946] Funding Source: Austrian Science Fund (FWF)
- Grants-in-Aid for Scientific Research [22370048] Funding Source: KAKEN
Planarial species are of especial interest to biologists due to the phenomenon of pluripotency and, in comparison to other developmental processes, it can be hypothesised that glycan-lectin interactions may play a role. In order to examine the N-glycans of one of these organisms, Dugesia japonica, peptide : N-glycosidase A was employed and the released glycans were subject to pyridylamination, HPLC and mass spectrometric analysis. A range of oligomannosidic glycans was observed with a trimethylated Man(5)GlcNAc(2) structure being the dominant species. Three glycans were also observed to contain deoxyhexose; in particular, a glycan with the composition Hex(4)HexNAc(2)Fuc(1)Me(2) was revealed by exoglycosidase digestion, in combination with MS/MS, to contain a galactosylated core alpha 1,6-fucose residue, whereas this core modification was found to be capped with a methylhexose residue in the case of a Hex(5)HexNAc(2)Fuc(1)Me(3) structure. This is the first report of these types of structures in a platyhelminth and indicates that the 'GalFuc' modification of N-glycans is not just restricted to molluscs and nematodes. Copyright (C) 2011 John Wiley & Sons, Ltd.
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