期刊
JOURNAL OF MASS SPECTROMETRY
卷 44, 期 1, 页码 50-60出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/jms.1470
关键词
N-Glycans; negative ion; electrospray; isomer differentiation; immunoglobulin G
资金
- Biotechnology and Biological Sciences Research Council Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Structures of N-glycans released from rat CEACAM1 expressed inhuman embryonic kidney cells were determined by MALDI and negative ion nanospray MS/MS techniques. The major carbohydrates were bi-, tri- and tetra-antennary complex glycans with and without sialic acid, fucose and bisecting GlcNAc residues. High-mannose glycans, predominantly Man(5)GlcNAc(2), were also found. The negative ion fragmentation technique easily identified the branching pattern of the triantennary glycans (mainly branched on the 6-antenna) and the presence of 'bisecting' GlcNAc residues (attached to the 4-position of the core mannose), features that are difficult to determine by traditional techniques. Sialic acids were in both alpha 2-3 and alpha 2-6 linkage as determined by MALDI-TOF MS following linkage-specific derivatization. Copyright (c) 2008 John Wiley & Sons, Ltd.
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