期刊
PARASITOLOGY
卷 142, 期 6, 页码 800-806出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182014001929
关键词
Cryptosporidium; Giardia; genotyping; subtyping; rodent
类别
资金
- State Key Program of the National Natural Science Foundation of China [31330079]
- National Natural Science Foundation of China [U1204328]
- Innovation Scientists and Technicians Troop construction Projects of Henan Province [134200510012]
- Program for Science and Technology Innovative Research Team in the University of Henan Province [012IRTSTHN005]
Cryptosporidium and Giardia are two important zoonotic intestinal parasites responsible for diarrhoea in humans and other animals worldwide. Rodents, as reservoirs or carriers of Cryptosporidium and Giardia, are abundant and globally widespread. In the present study, we collected 232 fecal specimens from commensal rodents captured in animal farms and farm neighbourhoods in China. We collected 33 Asian house rats, 168 brown rats and 31 house mice. 6.0% (14/232) and 8.2% (19/232) of these rodents were microscopy-positive for Giardia cysts and Cryptosporidium oocysts, respectively. All 14 Giardia isolates were identified as Giardia duodenalis assemblage G at a minimum of one or maximum of three gene loci (tpi, gdh and bg). By small subunit rRNA (SSU rRNA) gene sequencing, Cryptosporidium parvum (n = 12) and Cryptosporidium muris (n = 7) were identified. The gp60 gene encoding the 60-kDa glycoprotein was successfully amplified and sequenced in nine C. parvum isolates, all of which belonged to the IIdA15G1 subtype. Observation of the same IIdA15G1 subtype in humans (previously) and in rodents (here) suggests that rodents infected with Cryptosporidium have the potential to transmit cryptosporidiosis to humans.
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