期刊
PARASITES & VECTORS
卷 8, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13071-015-0869-3
关键词
Area-wide integrated pest management; Sterile insect technique; Pupae development; Low temperatures; Pupae transport; Mass-rearing; Diptera; Glossinidae
资金
- Government of Senegal
- Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture
- Department of Technical Cooperation of the IAEA
- CIRAD (Project Integrated Vector Management: innovating to improve control and reduce environmental impacts of Institut Carnot Sante Animale (ICSA) excellence network)
- US State Department
- SCAC of the Embassy of France in Burkina Faso
Background: The application of the sterile insect technique (SIT) requires mass-production of sterile males of good biological quality. The size of the project area will in most cases determine whether it is more cost effective to produce the sterile flies locally (and invest in a mass-rearing facility) or import the sterile flies from a mass-rearing facility that is located in another country. This study aimed at assessing the effect of long distance transport of sterile male Glossina palpalis gambiensis pupae on adult male fly yield. Methods: The male pupae were produced at the Centre International de Recherche-Developpement sur l'Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso, Burkina Faso, and shipped with a commercial courier service in insulated transport boxes at a temperature of +/- 10 degrees C to Senegal (+/- 36 h of transport). Upon arrival in the insectary in Dakar, the pupae were transferred to an emergence room and the flies monitored for 3-6 days. Results: The results showed that the used system of isothermal boxes that contained phase change material packs (S8) managed to keep the temperature at around 10 degrees C which prevented male fly emergence during transport. The emergence rate was significantly higher for pupae from batch 2 (chilled at 4 degrees C for one day in the source insectary before transport) than those from batch 1 (chilled at 4 degrees C for two days in the source insectary before transport) i.e. an average (+/- sd) of 76.1 +/- 13.2% and 72.2 +/- 14.3%, respectively with a small proportion emerging during transport (0.7 +/- 1.7% and 0.9 +/- 2.9%, respectively). Among the emerged flies, the percentage with deformed (not fully expanded) wings was significantly higher for flies from batch 1 (12.0 +/- 6.3%) than from batch 2 (10.7 +/- 7.5%). The amount of sterile males available for release as a proportion of the total pupae shipped was 65.8 +/- 13.3% and 61.7 +/- 14.7% for batch 1 and 2 pupae, respectively. Conclusions: The results also showed that the temperature inside the parcel must be controlled around 10 degrees C with a maximal deviation of 3 degrees C to maximize the male yield.
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