Lateral flow immunoassay using magnetoresistive sensors

Magnetic particles have been adapted for use as labels in biochemical lateral flow strip tests. Standard gold particle lateral flow assays are generally qualitative; however, with magnetic particles, quantitative results can be obtained by using electronic detection systems with giant magnetoresistive (GMR) sensors. As described here, these small integrated sensor chips can detect the presence of magnetic labels in capture spots whose volume is approximately 150 mu m x 150 mu m x 150 mu m. The range of linear detection is better than two orders of magnitude; the total range is up to four orders of magnitude. The system was demonstrated with both in direct and sandwich enzyme-linked immunosorbent assays (ELISAs) for protein detection of rabbit IgG and interferon-gamma, respectively, achieving detection of 12 pg/ml protein. Ultimately, the goal is for the detect or to be fully integrated into the lateral flow strip backing to form a single consumable item that is interrogated by a handheld electronic reader. (C) 2009 Elsevier B.V. All rights reserved.