期刊
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
卷 320, 期 19, 页码 2339-2344出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmmm.2008.04.160
关键词
maghemite; nanoparticle; surface functionalization; multifunctional nanoparticle; silicatein; protein separation
Multifunctional polymer-derivatized superparamagnetic iron oxide (gamma-Fe(2)O(3)) nanoparticles were prepared for biomagnetic separation of histidine-tagged recombinant proteins building up a faster and efficient method for protein separation by making use of their intrinsic magnetic properties. Using polymer bound gamma-Fe(2)O(3) nanocrystals, a 6 x histidine-tagged recombinant protein (silicatein) with a molecular weight of 24 kDa has been isolated and purified. The supermagnetic iron oxide nanocrystals were characterized by transmission electron microscopy (TEM), high-resolution TEM (HRTEM), SQUID and Mossbauer and the polymer functionalization of the gamma-Fe(2)O(3) nanocrystals was monitored by UV-vis spectroscopy and light microscopy. Protein immobilization and separation was monitored using immunostaining techniques and gel electrophoresis, respectively. (C) 2008 Elsevier B.V. All rights reserved.
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